人类乳头状瘤病毒与宫颈糜烂癌变的相关性研究

The Correlation between Human Papillomavirus andCervical Erosion for Carcinogenesis

目的 :探讨人类乳头状瘤病毒与宫颈糜烂和癌变的相互关系。方法 :阴道镜下对116例宫颈糜烂患者、12例宫颈癌患者及36例正常妇女宫颈 ,分别钳取活组织 ;用聚合酶链反应 ,对活组织进行HPV公共引物及HPV16、HPV18型特异引物检测。结果 :宫颈糜烂患者HPV阳性率为29.31 % ,正常妇女宫颈HPV阳性率仅11.11 %(P<0.05) ,宫颈颗粒型或乳突型糜烂与宫颈癌强相关(OR=5.56%CI=1.21～25.49)。“高危”HPV DNA检出率随宫颈鳞柱上皮异位程度增加而升高。结论 :宫颈糜烂癌变的病因可能与持续感染“高危”

Objective:To determine the correlation of human papillomavirus with cervical erosion and carcinogenesis.Methods:116 cases of cervical erosion and 12 cases of cervical cancer patients underwent cervical biopsy under colposcopy.HPV consensus primer and HPV16、HPV18 type specific primers were used to detect HPVs DNA in bisopy specimens from patients by polymerase chain reaction(PCR).36 normal cervical bisopy specimens were used and tested with the same method for control.Results:The result revealed that 29.31% of cervical erosions were positive with HPV consensus primers as compared with 11.11% of normal specimens (P<0.05)Our data suggested cervical erosion,especially the granular/papillar type was strongly associated with cervical carcinoma (OR=5.56 95% CI=1.21 25.49).The 'high risk' HPV16/HPV18 DNA was increasingly detected in cervical erosion while the squamous cell ectopy was increased.Conclusion:Persisting infection of 'high risk' HPVs may be related to cervical erosion and carcinogensis.