细胞凋亡在慢性心肌冬眠中的发生和作用

THE EFFECTS OF APOPTOSIS IN HIBERNATING MYOCARDIUM

目的 :探讨细胞凋亡在慢性心肌冬眠发生机制中的作用。方法 :将 2 2只犬冠状动脉左前降支内径缩窄约 50 % ,继续喂养 1个月 ,利用免疫组化法原位检测凋亡细胞 ,并利用琼脂凝胶电泳检测凋亡细胞的DNA片段。结果 :①成功地建立了慢性心肌冬眠的动物模型 ;② 10只犬存活 (45.2± 9.59)d ,左前降支内径缩窄平均 4 8% ,其中 4只犬的缺血标本中发现凋亡细胞及特征性DNA片段 ,且发现凋亡细胞在冬眠心肌中呈灶性分布。结论 :慢性冬眠心肌中存在细胞凋亡现象 ,但发生率较低。

Objective:To assess the effects of apoptosis in chronic hibernating myocardium.Methods:Stenosis of anterior descending coronary artery was created in 22 dogs and maintained for 45.2±9.59 days to reduced the diameter of coronary artery by 48% with severe regional myocardial systolic dysfunction.Documented by echocardiography,the animal model of chronic hibernating myocardium was established in 10 dogs.Apoptosis were detected with an in situ end labeling method and confirmed by deoxyribonucleic acid laddering on agarose gel electrophoresis.Results:①The animal model of chronic hibernating myocardium was successfully;②Apoptosis were found in 4 of 10 dogs and apoptosis cells presented a patchy distribution in hibernating myocardium.Conclusion:Myocyte apoptosis exists in hibernating myocardium,with lower rate.