尖锐湿疣患者HPV型与细胞免疫功能及复发关系的研究

HUMAN PAPILLOMAVIRUS TYPES AND THEIR CORRELATION WITH CELL MEDIATED IMMUNITY AND RECURRENCE IN PATIENTS WITH CONDYLOMA ACUMINATUM

目的 :研究尖锐湿疣患者人乳头瘤病毒 6 /11、16 /18型与T淋巴细胞亚群及复发的关系。方法 :聚合酶链反应检测皮损中HPV6 /11、16 /18,流式细胞仪检测T淋巴细胞亚群 ,用液氮冷冻、皮下注射转移因子及皮损局部注射干扰素治疗 ,疗程结束后随访 3个月。结果 :HPV6 /11感染 2 7例和HPV6 /11合并HPV16 /18感染 19例 ,治疗前外周血CD3、CD4 、CD4 /CD8均低于对照组 (P <0 0 1) ,CD8升高 (P <0 0 5) ,不同HPV型之间无差异。HPV6 /11感染组 8例复发 ,HPV6 /11合并HPV16 /18感染组 12例复发 ,二者差异有显著性 (P <0 0 2 5)。结论 :尖锐湿患者不同HPV型与复发有关。不同HPV型之间T淋巴细胞亚群无差异。

Objective:To study the correlation of human papillomavirus (HPV) type 6/11,16/18 and T cell subpopulation,recurrence in patients with condyloma acuminata (CA).Methods:Human papillomavirus type 6/11,16/18 in 53 CA samples was tested by polymerase chain reaction and T cell subpopulation in peripheral blood by flow cytometer.The patients were treated by crytherapy with liquid nitrogen,lesional injections of α interferon and subcutaneous injection transfer factor.The patients were followed up for three months after treatment.Results:The 53 patients with CA showed lower levels of CD 3+,CD 4+ T cell and CD 4+/CD 8+T cell ratio than those of the controls (P<0.01 ),and higher levels of CD 8+T cell( P<0.05). T cell subpopulation in 27 cases of HPV6/11+and 19 cases of HPV 6/11+with HPV 16/18+was similar.Eight patients with HPV 6/11+recured,tewlve cases with HPV 6/11+HPV 16/18+recured,and two groups had significant difference ( P< 0.025 ).Conclusion:The different HPV type with CA is related to the recurrence,but not related to T cell subpopulation.The T cell subpopulation of patients with CA before treatment is not related to recurrence.