复方解热镇痛药的毛细管胶束电动色谱快速分析

Fast determination of active ingredients in compound antipyretic analgesic tablets by micellar electrokinetic capillary chromatography

研究了用毛细管胶束电动色谱快速测定解热镇痛药复方阿司匹林 ( APC)中阿司匹林 ( AS)、非那西丁 ( PH)、咖啡因 ( CA)及复方扑热息痛 ( PAC)中扑热息痛 ( PA) ,AS,CA含量的方法 .使用长 37cm(有效长度 30 cm)内径 75μm的石英毛细管 ,+2 0 k V分离电压 ,在 p H8.65的 9mmol· L-1Na2 B4 O7-13mmol· L-1KH2 PO4 (内含 60 mmol· L-1SDS和 2 %乙醇 )缓冲溶液中 ,同一制剂中的三组分可在4 .5min内完全分离 .用紫外检测器在 2 14nm处检测 ,外标法定量 ,测定 4组分的相对标准偏差在0 .84 %～ 1.84 %之间 ,回收率在 99%～ 10 4 %之间 .所建立的方法应用于复方阿司匹林片和复方扑热息痛片的成分分析 。

A micellar\|electrokinetic\|capillary\|chromatographic approach (MECC) for fast determination of aspirin (AS), phenacetin(PH), caffeine(CA) and paracetamol(PA) in pharmaceutical preparations has been developed. Using a 37cm(effective length 30cm)~{!A~}75~{&L~}m i.d. fused\|quartz capillary and an applied voltage of 20kV, MECC is performed in 9 mmol\5L\+\{-1\} borax\|13 mmol\5L\+\{-1\} KH\-2PO\-4 buffer solution (pH8.65) containing 60 mmol\5L\+\{-1\} SDS and 2% alcohol. The four active ingredients are completely separated within 4.5 minutes, and quantified with UV\|detector at 214nm by external standard calibration. Relative standard deviation(RSD) for determining the four components holds in the range in 0.84%~{!+~}1.84%, while the recovery rate obtained in spike\|recovery test is in the range of 99%~{!+~}104%. The developed method can be successfully applied to the determining the active ingredients in compound aspirin tablet and compound paracetamal tablet.