甜菜丛生芽诱导及植株再生

Multiple Shoot Induction and Plantlet Regeneration in Sugarbeet

以 6个不同基因型的甜菜品种为试材 ,取其幼嫩叶柄、幼苗顶端生长点和幼嫩花序顶端切段做外植体 ,进行丛生芽诱导比较试验。试验结果表明 ,用花序切段作外植体产生丛生芽数量最多、速度最快。所有来自幼苗或生殖生长期植株的外植体产生的小苗均处于营养生长期。把这些快速生长的丛生芽分割成单芽继代培养能获得更多的丛生芽。

Taking 6 kinds of genotype sugarbeets as the experimental materials, with their petiole, seedling apical tips and inflorescence segments as explants, some experiments on multiple shoot induction were made. The results showed that the explants from the inflorescence segments produced the multiple shoots most and most rapidly and in all the 6 genotyopes. All the seedlings coming from the stage of seedlings or reproductive growth stage were in the vegetative growth stage. When the multiple shoots growing fast were cut into single shoot and subcultured, much more multiple shoots could be harvested.