棉铃虫精子在雌蛾生殖道内的转移动态

The transmission of sperm in the female reproduction tract of cotton bollworm, Helicoverpa armigera

通过显微镜观察、孚尔根涂片法和显微镜计数法研究了棉铃虫精子在雌蛾生殖道内的转移、分布及转移的动力。结果表明 :(1)交配结束后 1 5h ,雌蛾精包内的真核精子和无核精子开始向受精囊转移 ,2h时近 2 / 3的精子已转入受精囊 ,其后受精囊内精子的数量逐渐下降 ;(2 )精子转移至受精囊后只分布于主囊 ,成团聚集 ,副囊中没有精子 ;(3)雌虫生殖道肌肉的节律性收缩推动精子转移 。

The transmission and distribution of sperm in the reproduction tract of mated female Helicoverpa armigera ,were studied with Feulgen smear and microscope count methods.The results were summarized as follows:(1)Both eupyrene and apyrene sperms started their transmission from the spermatophore to spermatheca at 1 5 h after copulation,and finished this process in half hour.Thereafter,the number of sperms in spermatheca decreased gradually;(2)After transferred to spermatheca,the sperms stored in the utriculus in the form of sperm mass.No sperm was found in lagena;(3)Transportation of sperm to the spermatheca was achieved by means of rhythmic muscular contraction of the female reproduction tract.The active apyrene sperm was also believed to assist in the transmission of the eupyrene sperm.