检测猪链球菌2型毒力相关蛋白的ELISA法的建立

Establishing of ELISA for detecting virulence related proteins of Streptococcus suis type 2

提纯猪链球菌 2型分离株HA980 1的毒力相关蛋白溶菌酶释放蛋白 (MRP)和胞外因子 (EF) ,制备其抗体。用此抗体建立了Dot ELISA和间接ELISA检测法。分别以菌株ATCC35 2 46和HA980 1为阴性与阳性对照 ,检测 17株猪源链球菌和 1株猪链球菌 2型人分离株。结果显示 ,两种ELISA的检测结果一致 ,MRP和EF的阳性率均为 61% (11/18)。

Virulence related proteins of Streptococcus suis type 2,muraminidase released protein(MRP)and extracellular facter(EF)were extracted from isolate HA9801 and were used as antigens for preparation of antibodies.Dot ELISA and indirect ELISA were established for detection MRP and EF.17 strains swine Streptococcus and 1 human isolate of Streptococcus suis type 2 were detected,while strain ATCC35246 was used as negtive control and strain HA9801 as positive control.It showed that Dot ELISA and indirect ELISA appeared correspondent results. Positive rates of MRP and EF both were 61%.