Gus基因在转基因棉花细胞中的表达及其与NPTⅡ基因、Bt基因表达的相关性

Gus Gene Expression in Transgenic Cotton Cell and Its Correlation with NPTⅡ and Bt Genes

通过农杆菌介导法将含有 Gus基因、NPT 基因、Bt基因的 Ti质粒转入棉花细胞中 ,用Gus组织化学检测法检测转化愈伤组织、再生棉株 R0 、R1和 R2 代植株组织细胞中的 Gus基因表达的水平 ,并用 NPT 活性速测法和室内生物测虫法检测三个外源基因在 R0 、R1和 R2 代植物植株组织中的表达情况 ,分析三者存在及表达的相互关系。结果表明 ,Gus基因在转化愈伤组织细胞有丝分裂增殖过程中能稳定遗传给后代细胞。转化棉株 R0 、R1和 R2 代 Gus活性、NPT 活性、抗虫性三者有一定的相关性但不完全是共表达的 。

The plant cell transformation of cotton cultivars with plasmid containing Gus gene,NPTⅡ gene and Bt gene driven by CaMV 35S promoter was carried out by using Agrobacterium mediaum . The transgenic colli and leaf tissues of the putative transgenic cotton plants were treated with X glucuronide,and the transgenic tissue expressed the turned blue Gus gene.The results of Histochemical Gus assay suggested that the Gus gene was stably transmitted into the next generation cell in callus mitotic cycle. The Gus activity ,NPTⅡ and Bt activity of the progenies(R 1,R 2) of trasgenic cotton plants (R 0) showed the genetic correlation but partly coexpression,the expression of the three transgenes turn out to be heterogeneous.