卵巢癌组织中GST-π和MDR1基因的表达及其意义

Expression of GST-pi and MDR1 genes in operative specimens of ovarian cancer

目的 :观察两种耐药基因谷胱甘肽S 转移酶 pi(GST π)和MDR1在卵巢癌组织中mRNA水平的表达情况 ,探讨其表达的意义及应用价值。方法 :采用逆转录PCR方法检测了 18例卵巢癌和10例正常卵巢组织中GST π和MDR1的表达 ,应用 β 肌动蛋白作为内对照进行定量分析比较。 结果 :GST π和MDR1在正常卵巢组织中表达的阳性率分别是 2 0 %和 10 % ,在卵巢癌中的阳性表达率分别是 6 1.1%和 33.3%。GST π在癌组织表达高于正常组织 ,两者比较P <0 .0 5;而MDR1在癌组织表达虽高于正常组织但无统计学意义 ;在癌组织中有 4例同时出现了GST π和MDR1的共表达 ;GST πmRNA水平的表达低于蛋白水平的表达。结论 :(1)GST π和MDR1在卵巢癌组织中的表达均高于正常卵巢组织 ,但以GST π为主 ;(2 )GST π和MDR1在癌组织中的表达不一致 ,说明两者的作用机制不同 ;而共表达现象的存在 ,又说明GST π和MDR1可能有相同的调节因素存在 ;(3)GST πmRNA水平的表达低于蛋白水平的表达 ,提示GST π表达还存在转录后加工和 (或 )翻译水平的调控。

s] Objectives:To study the expression of GST pi and MDR1 genes in operative specimens of ovarian cancer,and to analyze the possible clinical role of GST pi and MDR1. Methods:Eighteen frozen specimens of ovarian carcinoma and ten specimens of normal ovarian tissues from patients were examined for the expression of GST pi and MDR1 genes by means of RT PCR, and quantitative analysis was performed using β actin as internal contrast.Results: Positive expression rate of GST pi and MDR1 in ovarian carcinoma were 61.1% and 33.3% ,respectively,and in contrast, 20% and 10% in normal ovarian tissues respectively. The level of GST pi gene expression in ovarian carcinoma was obviously higher than that in normal ovarian tissue (P< 0.05) and MDR1 gene also had high level expression in ovarian carcinoma, but had no statistical significantance. Four patients with ovarian carcinoma had GST pi and MDR1 coexpression. Expression levels of GST pi mRNA were lower than that of protein. Conclusions: (1) GST pi and MDR1 had higher level expression in ovarian carcinoma than in normal ovarian tissues. (2) GST pi and MDR1 may have same regulating factors but different mechanisms of action. (3)Processing after transcription and/or regulation of translation level may exist in GST pi expression. [