摘要
应用免疫细胞化学、流式细胞术和核酸原位杂交技术观测丹参酮 - A硫酸钠 ( DS2 0 1)对肺动脉平滑肌细胞( PASMC)增殖的影响。结果发现 :DS2 0 1能明显地阻抑 PASMC由 G0 /G1 期进入 S期 ( P<0 .0 5 ) ;使 PASMC增殖细胞核抗原 ( PCNA)的表达及 PDGF- A和 B链 m RNA的表达均显著降低 ( P<0 .0 1)。提示 :DS2 0 1可能通过下调 PDGFm RNA基因表达而有效抑制 HECCM诱导的 PASMC增殖。
Immunocytochemistry, flowcytometry and nucleic acid in situ hybridization were used to observe the effect of sodium tanshinon Ⅱ A silate (DS201) on proliferation of pulmonary artery smooth muscle cell (PASMC) induced by hypoxic endothelial cell condition medium (HECCM) The results showed that DS201 could obviously hinder PASMC to enter S phase from G 0/G 1 phase ( P <0 05), and decrease the expression of PCNA and platelet derived growth factor (PDGF) A and B chain mRNA in PASMC respectively, ( P <0 01) It was suggested that DS201 could probably inhibit the prolifertion of PASMC mediated by HECCM, through down regulating the expression of PDGF A and B chain gene
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第2期106-108,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学青年基金资助项目 ( No. 97BJ10 - 3 5 )
