摘要
为探讨能获得最佳转染效率和促增殖分化效应的 TGF-β1 基因转染条件、剂量及基因治疗的安全性 ,体外将TGF-β1 基因转入骨膜源间充质干细胞 ( MSCs) ,用水貂肺上皮细胞生长抑制法、透射电镜及软琼脂培养等方法检测。结果发现 :转基因 MSCs表达的 TGF-β1 具有生物学活性。当 3μl脂质体介导 1μg TGF-β1 基因转染时 ,能获得最佳转染效率和促 MSCs增殖及向成软骨方向定向分化效应 ;转基因细胞无恶性转化倾向。从而把组织工程学与分子生物学有机结合成为分子组织工程学 ,为高质量地修复关节软骨缺损奠定了良好的基础。
The conditions for high efficient transforming growth factor (TGF) β 1 gene transfer and its effect on the bioactivity of mesenchymal stem cells (MSCs) were studied to provide a basis for accelerating articular cartilage repairing The principles of tissue engineering were combined organically with the principles of gene therapy to produce cultured MSCs transfected with the full length rat TGF β 1 cDNA in vitro The bioactivity and safety of the transfected MSCs were examined The results showed that the effect of TGF β 1 gene transfer on the bioactivity of MSCs depended on the tranfection conditions The high efficient expression and best promotion effect were achieved when the amount of TGF β 1 cDNA was 1 μg and the amount of lipofectamine was 3 μl There was no clone formation when the transduced MSCs cultured in bilaminar agarose It was concluded that the bioactivity of MSCs was enhanced after it transfected with gene encoding TGF β 1 This new gene therapy protocol also possesses safety and feasibility for the repairing of damaged articular cartilage, especially those caused by diseases during the advanced stages
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第2期125-128,共4页
Acta Universitatis Medicinae Tongji
基金
国家重点科技攻关资助项目 ( No.9692 0 2 0 11)
