摘要
12例垂体瘤做原代细胞培养 ,检测了表皮生长因子 ( EGF)及染料木黄酮 ( Genistein)对肿瘤细胞 3H- Td R掺入率及酪氨酸激酶产物表达的影响。研究表明 EGF使大部分功能性腺瘤及所有非功能性腺瘤细胞的 3H- Td R掺入率明显增加 ,酪氨酸激酶活性增强 ,而 Genistein能抑制 EGF的作用使所有瘤细胞的 3H- Td R掺入率下降。提示表皮生长因子对垂体瘤细胞有明显的增殖刺激作用 ;Genistein能消除表皮生长因子的细胞增殖刺激作用 ,抑制肿瘤细胞增生 ,对垂体瘤治疗有重大意义。
Recombinant human epidermal growth factor (rhEGF, 20 ng/ml) and Genistein (740 μmol/ml) were added to the cells from 12 cases of human pituitary tumors and co cultured in vitro The 3H thymidine uptake of tumor cells was measured by scintillation counter and tyrosine kinases activity was detected by using immunocytochemical method The results showed that 3H thymidine uptake and tyrosine kinases activity were increased in 10 out of 12 cases of human pituitary tumor by EGF and both declined by Genistein It was suggested that EGF had a strong mitogenic effect on majority of pituitary tumors Genistein could abolish the effects of EGF promoting the proliferation of the tumor cells, inhibit the growth of the tumor and might be useful in pituitary tumor treatment
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第2期131-133,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金资助项目 ( No.3 9670 73 6)
