从妊娠人胎盘绒毛组织克隆HLA-G1 cDNA

Cloning of HLA-G1 cDNA from the First Trimester Placental Tissue

为系统研究 HL A- G1分子的生物学功能 ,采用 RT- PCR技术 ,从人流的胎盘绒毛组织中提取总 RNA,经过逆转录合成 HL A- G1的全长 c DNA,插入到真核表达载体 pc DNA3中 ,构建重组表达质粒 pc DNA3- HL A- G1;转化大肠杆菌 DH5 α,筛选其阳性克隆株 ,经酶切、 PCR及测序鉴定 ,证实 HL A-

In order to systematically study the function of HLA G1 molecule, total RNA was extracted from the first trimester trophoblast and the full length HLA G1 cDNA was prepared by using the technique of RT PCR. Recombinant of HLA G1 cDNA and plasmid pcDNA3 was constructed, and then transformed into E. coli DH5α. The recombinant was confirmed by restriction enzyme analysis and PCR analysis. The result of DNA sequencing showed that the pcDNA3 HLA G1 recombinants had successfully been constructed.