金丝桃抗脂质过氧化作用研究

Studies on the Effect of Hypericum Chinense on Antilipid-Peroxidation

为研究金丝桃的抗脂质过氧化作用 ,用分光光度法测定了金丝桃提取物对大鼠离体组织匀浆的脂质过氧化及由 Fe2 + - H2 O2 体系、Fe2 + - Vit C体系和 Fe2 + - Cys体系诱导的脂质过氧化的抑制作用。发现金丝桃提取物对大鼠肝、心、肾组织匀浆脂质过氧化和线粒体诱导的脂质过氧化具有抑制作用并表现出剂量依赖关系 ,而且提取物 F0 0 3的抑制作用要强于 F0 0 4、 F0 0 5的抑制作用。认为金丝桃具有较好的抗脂质过氧化作用 。

The antilipid peroxidation of the extracts of Hypericum Chinense was studied. The antilipid peroxidation of Hypericum Chinense on liver, kidney, heart and mitochondria was measured by the determination of malondialdehyde (MDA) using TBA spectrometric method. The results showed that the extracts of Hypericum Chinense could inhibit the lipid peroxidation of liver, kidney, heart tissue homogenate and the lipid peroxidation of mitochondria induced by Fe 2+ H 2 O 2 , Fe 2+ VitC and Fe 2+ Cys. The inhibition exhibited concentration dependent manner. The inhibitory effect of the extracts of F003 was stronger than that of the extracts of F004 and F005. It was suggested that Hypericum Chinense had a good antilipid peroxidation effect, therefore it may be necessary to study it further.