SRPK2的测序及分析

Sequencing and Analysis of Serine/Arginine Protein-Specific Kinase

用 SRPK1有关的克隆 ,制备探针 ,并与人胎脑 c DNA文库进行筛查。测得 SRPK2 3.744 kb的核苷酸序列 ,并与 SRPK1比较。显示 SRPK2与 SRPK1的序列有 77%的相近 ,而在 SRPK1激酶区域有 90 %相似。从 SRPK2的氨基酸序列上看 ,其 NH2 端存在一段富含脯氨酸序列区。推测 SRPK2是 SRPK家族的新成员 ,SRPK2的 NH2 端的富含脯氨酸结构可使其具有独特的调节功能 。

Serine/arginine protein specific kinase (SRPK1) was cloned to prepare probes. All 8 SRPK1 related EST cDNA clones were sequenced by using Erase a Base. DNA probes derived from one clone were used to screen a human fetal brain cDNA library in the Lamda ZAP II vector. A longest clone, 3.744 kb, was sequenced in both strands. The results showed that SRPK2 displayed 77 % identity and 90 % similarity to SRPK1 over their entire kinase domains. In addition, SRPK2 contained a stretch of proline rich sequence at the N terminus. It was concluded ed that SRPK2 might be a new SRPK family member with its sequence similar to SRPK1, suggesting both SRPK2 and SRPK1 might have similar enzymatic activity and substrate specificity. The N terminal proline rich motif might function as a targeting signal to interact with substrates and (or) regulators.