反复听源性惊厥对大鼠各脑区葡萄糖摄取率的影响

The Effects of Repeated Audiogenic Seizures on Cerebral Glucose Utilization in Audiogenic Seizure Susceptible Rats

采用~3H-2-脱氧葡萄糖(~3H-2-DG)定量方法研究大鼠听源性发作后各脑区葡萄糖摄取率的变化。实验动物每天在强声(115±5dB)刺激下诱导发作一次,共五次发作;对照动物为未经强声刺激的易感性大鼠。第六天,实验动物和对照动物都在安静的状态下测定其各脑区的糖代谢率。结果表明反复听源性发作之后,几乎所有的脑区都出现糖代谢率增高(p<0.05),其中下丘、上撖榄核、外侧丘系核、上丘增高更明显(p<0.01)。提示这些脑区可能与大鼠听源性发作的起源及传播途径有关。

The experiments were carried out on P77-PMC audiogenic seizure (AGS) susceptible rats. Six female rats were divided into two groups: experimental and control group. Experimental rats were treated AGS once daily for 5 days. All the AGS score were 2 according to scoring system. Control rats were seizure naive rats which did not receive any intense sound stimulation. Local cerebral glucose utilization (LCGU) meazurements 晈ere performed on sixth day. After repeated AGS, almost all of the 18 brain regions examined exhibited increased rates of LCGU than control (P<0.05) . Among them, inferior colliculus, superior olivary nucleus, nucleus lateral lem-niscus, superior colliculus had more higher LCGU rates (P<0.01) . The results suggest that these structures may involved in the audiogenic seizures in rats.