原发性肝细胞癌及癌旁组织中环氧合酶2的表达及其意义

Significance of cyclooxygenase-2 expression in human primary hepatocellular carcinoma and adjacent nontumorous tissues

目的 研究人原发性肝细胞癌 (HCC)组织和癌旁非肿瘤组织中的环氧合酶 2 (COX 2 )蛋白及基因表达情况。方法 采用免疫组化法和原位分子杂交法 ,研究 2 7对原发性肝细胞癌及癌旁非肿瘤组织、5例正常肝组织中COX 2的蛋白和基因表达。结果 高分化HCC中COX 2蛋白表达显著高于中分化和低分化HCC(P分别 <0 .0 5 )以及癌旁组织和正常组织 (P分别 <0 .0 1) ;癌旁组织的COX 2表达显著高于正常组织 (P <0 .0 5 ) ;癌旁组织、中分化和低分化HCC之间COX 2的表达强度差异无显著性 (P >0 .0 5 )。在COX 2蛋白阳性的肝癌细胞和癌旁肝细胞胞质中可见到COX 2mRNA呈阳性表达。结论 COX 2的过度表达可能参与了高分化HCC的致癌过程。

Objective To study significance of cyclooxygenase 2 (COX 2) expression in human primary hepatocellular carcinoma (HCC) and adjacent nontumorous tissues. Methods The COX 2 protein and mRNA were investigated in 27 HCC tissues and corresponding adjacent nontumorous tissues, and 5 histologically normal liver tissues, using immunohistochemistry and in situ hybridization. Results The well differentiated HCC expressed COX 2 protein stronger than moderated HCC and poor differentiated HCC ( P <0.05 respectively), and adjacent nontumorous tissues and normal liver tissues ( P <0.01 respectively); more intensive staining of COX 2 in adjacent nontumorous tissues rather than normal liver tissues was observed ( P <0.05). There was no significant difference among adjacent nontumorous tissues, moderated HCC and poor differentiated HCC ( P >0.05). The expression of COX 2 mRNA was observed in the plasma of the cells in HCC and the hepatocytes in adjacent nontumorous tissues which COX 2 protein were positive. Conclusion The result of the present study suggests that COX 2 may involve in carcinogenensis of well differntiated HCC.