卵巢切除及高脂饮食对雌鼠心脏组织、血管内皮细胞雌激素受体含量的影响

The effects of ovariectomy and cholesterol rich diet on the content of estrogen receptor in vascular endothelial cells and hearts of female rats

目的 探讨卵巢切除 (去势 )、高脂饮食对雌鼠心脏组织和肺血管内皮细胞中雌激素受体 (ER)含量的影响。 方法 用放射配体结合法分别测定去势、高脂饮食及雌激素替代治疗后雌鼠心脏、血管内皮细胞中ER含量 ,并对雌鼠去势前后血清雌二醇及血脂水平进行检测。 结果 心脏及血管内皮细胞中ER含量在去势后〔(0 5 1± 0 0 9)fmol/mgpro ,(6 73± 0 5 2 )fmol/ 10 6cell〕及高脂饮食后〔(0 97± 0 12 )fmol/mgpro ,(9 15± 0 5 3)fmol/ 10 6cell〕均较假手术组〔(2 0 8± 0 15 )fmol/mgpro ,(17 6 6± 1 2 6 )fmol/ 10 6cell〕 ,显著下降 (P <0 0 1) ,但以去势后为明显 ;补充雌激素后ER表达得到明显改善〔(1 75± 0 2 8)fmol/mgpro ,(13 81± 1 0 6 )fmole/ 10 6cell〕 ;去势组雌鼠血清雌二醇水平较假手术组明显下降〔(181± 36 )pmol/L对 (10 0 4± 88)pmol/L ,P <0 0 1〕 ,胆固醇水平显著升高〔(1 84± 1 2 7)mmol/L对 (1 2 2± 0 11)mmol/L ,P <0 0 1〕。 结论 雌激素水平明显影响雌鼠心脏组织和肺血管内皮细胞中雌激素受体的含量 ,高脂饮食可明显降低组织和细胞中雌激素受体的含量。

Objective To investigate the effects of ovariectomy and cholesterol rich diet on the change of estrogen receptor content of vascular endothelial cells and hearts of female rats. Methods The receptor binding assay (RBA) was adopted to measure the content of estrogen receptors, and the serum levels of estradiol and lipids were measured. Results The content of ER was much lower in hearts and VEC of ovariectomy〔(0.51±0.09) fmol/mg pro,(6.73 ± 0.52) fmol/106 cell〕and cholesterol rich rats〔(0.97±0.12) fmol/mg pro,(9.15 ±0.53) fmol/106 cell〕than pseudo-operation rats 〔(2.08±0.15) fmol/mg pro ,(17.66±1.26) fmol/106 cell , P<0.01〕, but more apparently at the group of ovariectomy. Given estrogen for nine weeks, the content of ER was improved〔(1.75±0.28) fmol/mg pro,(13.81±1.06) fmol/106 cell〕. Serum level of estradiol dereased 〔(181±36) pmol/L vs.(1 004±88) pmol/L, P<0.01〕 and serum cholesterol level increased 〔(1.84±1.27) mmol/l,(1.22±0.11) mmol/l, P<0.01〕 significantly after the ovaries of female rats were resected. Conclusions The content of ER in VEC and hearts are affected by serum estrogen level, and cholesterol rich diet decrease the content of ER remarkably.