摘要
免血清先经硫酸右旋醣酐沉淀、浓缩HDL,然后再超速离心分离HDL,经脱脂后再进行大柱床体积Sepha-dex G-150凝胶柱层析,较大批量地分离apoA-I,一次可获碍纯品数百毫克,经鉴定为apoA-I.可满足实验室或临床对apoA-I大量使用的需要。
Rabbit sera were firstl precipitated and concentrated by Dextran sulphate. After further isolation by ultracenlrif ugation, HDL were delipided and applied to a large column ( 4.2 × 180cm ) of Sephadex G-150 for separation of apoA-I. Approximately three hundred milligrams of purified apoA-I would be harvested in each process, which were identified by SDS PACE, immunodif fusion and LCAT activity assay. .
出处
《基础医学与临床》
CSCD
1991年第5期57-60,共4页
Basic and Clinical Medicine
