一种脑代谢研究的有效方法——高分辨率磁共振波谱分析

A POWERFUL METHOD OF STUDYING METABOLISM IN BRAIN——High Resolution Nuclear MagneticResonance Spectroscopy

介绍了一种脑代谢研究的有效方法 .成年大鼠脑经漏斗法液氮冷冻 ,制备脑组织高氯酸提取物并冷冻干燥 ,所得固体溶于D2 O后用1 H和31 P磁共振波谱 (MRS)检测 .结果表明 :脑高氯酸提取物的磁共振波谱有着极好的分辨率 ,31 PMRS可以分辨出ATP、ADP、磷酸肌酸(PCr)、无机磷以及多种磷酯和糖磷 ;1 HMRS可以分辨出乳酸 (Lac)、N 乙酰天冬氨酸 (NAA)、胆碱 (Cho)、肌酸 (Cr)、GABA、肌醇 (Ino)、琥珀酸 (Suc)以及多种氨基酸 .各波峰积分后得到各种物质的相对含量 ,而这些代谢中间产物的相对含量变化可以反应脑内的代谢状况和脑受损伤情况 .

In this paper, a howerful method was introduced to study the metabolism in brain. Adult rats were frozen with liquid-N-2 through a self-command funnel. After perchloric acid (PCA) extracts were prepared and lyophilized, the residual substance were dissolved in D-2O and investigated with {}+1H and {}+{31}P nuclear magnetic resonance spectroscopy (MRS). Result:High-resolution nuclear magnetic resonance spectroscopy of brain tissue was achieved. {}+{31}P MRS reveals ATP,ADP,phosphocreatine, inorganic orthophosphate and manifold aliphatic phosphates and sugar phosphates; {}+1H MRS shows many resonance peaks, including lactate, N-acetylaspartate, choline, creatine, GABA, myo-inositol, succinate and manifold amino acids. The peaks were integrated and the relative values might be used to express the metabolism or damnification of brain.;