摘要
黑曲霉 (Aspergillusniger)固态发酵后粗酶液经硫酸铵盐析 ,2次SephadexG - 2 0 0柱层析后可提纯8倍左右。CMC酶最适作用温度为 60℃ ,最适作用pH为 3 5,30℃～ 70℃区间酶活力较稳定 ,在pH3 0～5 0范围内 ,50℃保温 30min能保持 80 %的酶活力。CMC酶的Km、Vmax值分别为 7 69%CMCg/ml、0
Cellulose from Aspergillus niger was purified by (NH 4)\-2SO\-4 precipitation,twice Sephadex G-200 column gel filtration.The cellulase activity was greatly enhanced.The optimum condition for cellulase activity were temperature 60℃,pH3.5.It showed a high stability at temperature 50℃,pH3.0- 5.0 .Km and V max value were 7.69% and 0.33 mg/ml.min respectively.
出处
《生物技术》
CAS
CSCD
2001年第3期7-9,共3页
Biotechnology
基金
南京市科委资助项目 (宁科 1999- 992 2 1)
