一株海洋细菌产碱性蛋白酶的研究

Study on the production of an alkaline protease produced by a marine bacterium

对 1株产碱性蛋白酶的海洋细菌Pseudomonassp .7- 11发酵产生碱性蛋白酶的条件和酶的调控机制进行了初步研究。结果显示 ,7- 11为好氧菌 ,具有某些嗜低温特性 ,且适宜在偏碱性的条件下生长并产酶 ,该菌产生碱性蛋白酶表现出初级动力学特征 ;酪蛋白培养基适合菌株产酶 ;K+对于菌株产酶有关键作用 ;复合氨基酸对产酶有抑制作用 ;初步显示 ,7- 11产生蛋白酶受诱导及阻遏两种方向的调控。

The fermentation conditions of the alkaline protease produced by a marine bacterium strain 7-11 and the regulation mechanism of the protease production was studied.The study showed that 7-11 was aerobic and psychrophilic.The curve of alkaline protease production showed primary metabolite kinetics.the casein medium improved the growth of the organism and the production of the protease.K\++was vital for the enzyme production.Composite amino acid inhibited the production of the protease.