利用精子介导法向蚕卵导入外源基因的研究

Sperm-mediated Gene Transformation of Silkworm

为建立家蚕转基因中切实可行、操作简便的外源基因导入方法 ,进行了精子介导法探索 ,以精子介导法的三种方式向家蚕导入所构建质粒 pFbGFP ,并通过PCR扩增和DNA印迹等手段 ,已连续两代从基因组DNA检测到导入外源基因GFP的存在 ,其中的一种导入方式到第二代阳性率约 30 % .结果表明该法可有效进行家蚕转基因的外源基因导入 .

To find practical and simple transformation methods in transgenic silkworm research, the sperm mediated methods were tried. The constructed plasmid pFbGFP was transformed into silkworm( Bombyx mori ) with three different sperm mediated transformation methods. Positive results of PCR and Southern blotting was screened in the following two generations. 30% positive rate of the second generation was obtained by one of the methods. This result showed that this was an effective method for transformation of silkworm with foreign gene.