南京市正常人群NQO1、CYP1A1、mEH基因的多态性研究

Polymorphisms of NQO1, CYP 1A1, mEH Genes in Normal Nangjing Population

应用PCR技术 ,对南京市正常人群中NQO1、CYP1A1、mEH -外显子 3、mEH -外显子 4基因型多态性进行了研究。 88例样本中 ,相关基因野生型纯合子 (wt/wt)、杂合子 (wt/vt)、突变型纯合子 (vt/vt)三种基因型的频率分布及基因频率分别是 :NQO12 9.5 % (0 .30 4) ,5 1.1% (0 .495 )和 19.3% (0 .2 0 2 ) ;CYP1A135 .2 % (0 .32 9)、44 .3% (0 .489)和 2 0 .5 % (0 .181) ;mEH -外显子 3为 2 6 .1% (0 .2 97) ,5 6 .8% (0 .496 ) ,17.0 % (0 .2 0 7) ;mEH-外显子 4为 83.0 % (0 .82 6 ) ,15 .9% (0 .16 5 ) ,1.1% (0 .0 0 8)。以上结果与国外的有关报道存在一定差异 ,在不同地区中国人群的频率分布特征基本一致 。

The polymorphisms of NQO1, CYP1A1, mEH -Exon3,and mEH -Exon4 genes in normal Nanjing population (88 cases) were investigated by PCR approach. The results showed that the population frequency distributions of genotypes of wild-type,heterozygote, homozygous variant were respectively: NQO1 29.5%,51.1%,19.3%; CYP1A1 35.2%,44.3%,20.5%; mEH -exon3 26.1%,56.8%,17.0%; mEH -exon4 83.0%,15.9%,1.1%. The frequency distributions of genotypes in Nanjing population differ from those of other countries and do not show marked differences compared with other different area in Chinese population. The ethnic difference might be an important reason which results in the differences of related genotypes.