2-(4′-噻唑)苯丙咪唑对猪脑微管蛋白体外聚合的影响

Studies of Tubulin Polymerization in vitro Affected by Thiabendazole

本研究利用猪脑中分离纯化的微管蛋白聚合和解聚反应 ,分析了具有争议的非整倍体诱发剂 2 (4′ 噻唑 )苯丙咪唑 (thiabendazole ,TBZ)对微管蛋白聚合状态的影响。秋水仙素 (colchicine)为本研究的阳性对照物。结果发现 :2 (4′ 噻唑 )苯丙咪唑能显著抑制体外微管蛋白的聚合 ,并呈明显的剂量效应关系。研究表明 ,TBZ可能通过抑制微管蛋白聚合来影响染色体正常分离 。

Tubulin isolated from porcine brain was employed in the experiment to study the mechanism of aneuploidy induced by thiabendazole(TBZ) by analyzing the polymerization of tubulin. Colchicine was set as a positive control of the experiment. The experiment indicated that TBZ could inhibit the tubulin polymerization significantly and showed a dose effect relationship. The result suggested that TBZ may induce aneuploidy by the pathway of tubulin polymerization inhibition which is able to lead to chromosomal malsegregation.