云南红豆杉天然群体内同工酶遗传变异的研究

Genetic Variation of Isoenzyme within Natural Population of Taxus yunnanensis in Yunnan of China

采用水平淀粉凝胶电泳技术 ,对分布于金沙江流域的云南红豆杉天然群体的 10种酶系统同工酶的遗传变异进行了研究。在谱带遗传分析的基础上确定了 15个酶基因座及其等位基因。其中有 14个酶基因座属多态 ,只有一个单态基因座 (ME - 3)。 14个多态基因座中 ,4个基因座遗传变异小 ,对该天然群体的遗传变异贡献不大 ,其余 10个基因座遗传变异丰富 ,对该天然群体的遗传变异贡献大。该天然群体具有明显丰富的遗传变异性 ,多态基因座比率P =0 .933,等位基因平均数A =2 .90 ,平均期望杂合度He=0 .2 90。

Genetic variation of ten isoenzymes was studied within population of Taxus yunnanensis Cheng et L. K. Fu in the Jinsha River Valley using the method of horizontal starch gel electrophoresis.On the basis of banding analysis,eight enzyme systems , presumablly coded by fifteen isoenzyme loci and their alleles were scored , and fourteen were polymorphic with only one monomorphic locus( ME-3 ). Among them , four polymorphic loci with inevident genetic variation made little contribution to genetic variation of this population and other ten polymorphic loci with evident genetic variation made great contribution to genetic variation of this population . Isoenzyme data indicated high level of genetic variability in this population with P=0.933,A=2.90,He= 0.290 . The taxol content had close relation to genetic variation of population.