摘要
本文对云南首次分离到的Sindbis病毒进行了滤过试验、耐酸耐醚试验、致细胞病变、动物敏感性试验、血凝特性、空斑和毒力等试验研究 ,结果符合披膜病毒科的病毒特性。交叉血抑试验和免疫荧光试验 ,以及空斑减少中和试验进一步证实为甲病毒属的Sindbis病毒。其空斑纯化株的生物学特性也与原株相符 ,纯化株的制备为该株病毒分子生物学研究的准确性和一致性提供了条件。云南Sindbis病毒的首次分离具有重要的流行病学意义 ,其生物学特性研究结果对我省该病的诊断和防治具有重要的指导意义。
The biological characters of Sindbis virus strain of Yunnan(YN87448 strain) were studied by the test of the filtration, acid resistant, ether resistant, CPE, susceptibility of animal, HA, plague, determination of virus titres, and the cross HI, cross IFAT and PRNT as well.The results indicated that YN87448 strain belongs to Sindbis virus, Alphavirus, Togaviridae. YN87448 strain virus was plaque purified(PYN87448). The biological character of PYN87448 strain virus was studied too. PYN87448 strain virus will be used in the molecule biological test.
出处
《中国病毒学》
CSCD
2001年第2期97-100,共4页
Virologica Sinica
