青霉菌立体选择性环氧化顺丙烯磷酸产生磷霉素

THE EPOXIDATION OF cis-PROPENYLPHOPHONIC ACID TO FOSFOMYCIN BY PENCILLIUM SP.

由土壤中分离出一株青霉 (Penicilliumsp .) ,编号F5,能选择性的将顺丙烯磷酸环氧化为磷霉素 ,在pH7 5、2 8℃、2 80r min条件下培养 6d ,底物浓度 0 3%时 ,产物浓度达 2 2mg mL ,产率 41 % ;底物浓度 0 6%时产率 8%。转化产物经磷霉素敏感菌生物检测 ,TLC检测 ,并与标准品比较 ,确证为磷霉素。

One strian of Penicillium sp.F5,found from soil sample,was able to stereo\|specific epoxidation of cis\|propenylphosphonic acid(PPOH) to fosfomycin\[(\|)\|(1R,2S)\|1,2\|epoxypropyl\|phosphonic acid,FOM\] during the cultivation.The product (FOM) was identified by thin layer chromatography and microbiological assay.Under the culture conditions of 28℃ pH7.5 and 280r/min, 0.3% concentration of PPOH,reached a level of 2.2 mg/ml of FoM and the yield was 41%.When the PPOH concentration was 0.6%,the yield was 8%.