摘要
对黑柄炭角菌深层发酵制品中的DPPH自由基捕捉成分进行研究。经硅胶柱层析、中压液相色谱顺相和反相分离、制备型高压液相色谱分离等一系列步骤 ,共获得相对纯度在85%以上 ,收量在 2mg以上的自由基捕捉物质 2 0个 ,对其中的B4 1 6进行了质谱、1H NMR、13C NMR、1H 13CHMBC、红外光谱等的测定 ,测得分子式为C10 H10 O4 ,推断它为 5,8二羟基 3 甲基 3,4二氢异香豆素。在 2 0 μmol L时 ,它的DPPH自由基捕捉活性为维生素C的 1 67倍 ,维生素E的 2 1倍。
The DPPH free\|radical scavengers from the fungal Chinese medicine `Wulingshen' ( Xylaria nigripes ) were studied.After separation using silica gel colunm chromatography,MPLC and HPLC,20 DPPH free\|radical scavengers with purity higher than 85% and yield more than 2 mg were screened,in which compound B4\|16 had relatively higher yield and stronger free radical scavenging activity.The formula of compound B4\|16 was determined to be C 10 H 10 O\-4(MW:194)based on its FAB\|MS, 1H\|NMR, 13 C\|NMR, 1H\| 13 C HMBC,IR spectra,and its structure was elucidated.It was identical with 5,8\|dihydroxy\|3\|methyl\|3,4\|dihydroisocoumarin.At the concentration of 20 μmol/L,its DPPH free\|radical scavenging activity was as 1.67 times as that of vitamin C or 2.10 times as that of Vitamin E.
出处
《微生物学报》
CAS
CSCD
北大核心
2001年第3期363-366,共4页
Acta Microbiologica Sinica
基金
RotaryInternational资助
