子午沙鼠感染戊肝病毒传代的实验研究

Study of Experiment on HEV Infection in Gerbil(Meriones meridianus)from Generation to Generation

目的 :子午沙鼠实验感染HEV的传代研究。方法 :选健康子午沙鼠腹腔接种含HEV的人粪便悬液 ,接种后观察 43～ 5 0d ,分别记录临床表现、酶血症和排毒结果、肝组织学改变。第 1代 7只 ,第 2、3代各 2 0只 ,依次进行逐代感染 ,连续 3代。结果 :临床表现不明显 ,酶血症反应 3代基本一致 ,第 6d开始 ,5 0d渐复常 ,呈双峰型 ;肝组织学改变 :经光镜和电镜检查显示 ,与人戊肝病理改变相似。结论 :实验证实子午沙鼠可连传 3代 ,有与人类戊肝相似的特征 。

Objective: Test of HEV infection in Meriones meridianus step by step in generation. Method: The suspention liquid of waste of man,who was HEV patient,were injected into the abdominal cavities of healthy M.meridianus which were observed in 43-0 days afte injected.It was recorded for the clinical characters,Blood-enzyme,results of wasting HEVRNA,and the changes of liver's histology.The 7 bodies in the first time and 20 bodies both the second and third were injected step by step in generation respectively. Result: There were indistictively clinical characters,and similar reactions of blood-enzyme in all generations.There were two peak models of blood-enzyme from the 6 day to the 50 day after injectsd. Comparison of changes in liver histology was similar between the gerbils infected by HEV and the HEV patients. Conclusion: The experiments shown that gerbil can be infected by HEV step by step in three generations,in which the clinical characters is similar to HEV patients,and considered as laboratory animal model for HEV.