摘要
参照人SRYgeneHMG -box保守区的序列 ,设计一对引物 ,扩增了乌龟的Soxgene,并对扩增条件进行了优化。结果显示乌龟Soxgene的扩增片段与人SRYgene扩增片断大小相同 ,为 2 2 0bp左右 ,且雌雄个体间无差异 ;最佳扩增条件为 :Mg2 +2 .0mM、dNTP 12 0 μM、引物 0 .3μM及退火温度 5 2℃。本研究为探索乌龟的性别决定机制以及Soxgene进化的保守性提供分子资料。
The sox gene of Chinemys reevessi was amplified by using a pair of primers which could amplify the conservative motif of human SRY gene. The amplification band with the length of 220bp was observed in both male and female Chinemys, which was consistent with that of human SRY gene. The optimization system of PCR reaction was 2.0 mM Mg 2+ ,dNTP 120μM,0.3μM primer and annealing at 52℃. This research presents molecular proof for the mechanism of turtle's sex determination and the conservation of sox gene's evolution.
出处
《四川动物》
CSCD
2001年第2期82-85,共4页
Sichuan Journal of Zoology
基金
安徽省自然科学基金
安徽省教育厅基金
