用去透明带仓鼠卵穿透试验检测牛冷冻精子的受精能力

Detection of the fertilization ability of bovine frozen-thawed sperm by penetrating the zona-free ova of hamster

研究了用去透明带仓鼠卵穿透试验检测牛冷冻精子的受精能力。对 36头西门塔尔、 2 3头夏洛来和 12头利木赞牛冷冻精子的实验结果表明 :三个品种的牛冻精均能穿入去透明带仓鼠卵 ,并形成发育良好的雄原核。穿透率在 70 %～ 80 %左右 ,有原核卵百分率在 30 %左右。

This paper investigated the fertilization ability of bovinc frozen-thawed sperm by penetrating the zona-free ova of hamster.We detected the frozen-thawed sperm of 36 Simmental,23 Charolais and 12 Limousin,the results showed that the frozen-thawed sperm of these three breeds could penetrate the zona-free ova of hamster,and formed male pronucleuses.The penetrating rate was 70%～80%,and the rate of pronucleuses formation was about 30%.Conclusion:this method is valid for detecting the fertilization ability of frozen-thawed sperm.