摘要
Lectin binding patterns in the hemopoietic organs and blood cells of Grass carp ( Ctenopharyngodon idellus ), Chinese soft shelled turtle ( Trionyx sinensis) were observed with two affinity histochemical methods, ABC staining and fluorescence bound lectin staining. Concanavalin (ConA), Phytohemagglutinin (PHA), Pokeweed agglutinin (PWM) and Lipopolysaccharide (LPS) binding sites were distributed mainly on the plasma membrane of lymphocytes. ConA, PHA binding sites were distributed in thymus, cordic structure of head kidney and scatterly spleenic tissue of C. idellus, also in thymus and periellipsoidal lymphatic sheaths and marginal region of T. sinensis. PWM, LPS binding sites were scatterly distributed in cordic structure of head kidney of C. idellus, spleenic tissue of C. idellus and T. sinensis, but positive cell groups were not obvious. ConA, PHA and PWM positive percents of lymphocytes in blood smears were repectively 36%~41%, 33%~37%, 38%~43% in C. idellus and 37%~48%, 36%~45%, 44%~53% in T. sinensis, but percents of positive cells were repectively 15%~18%, 14%~17%, 9%~12% in imprints of head kidney of C. idellus.
Lectin binding patterns in the hemopoietic organs and blood cells of Grass carp ( Ctenopharyngodon idellus ), Chinese soft shelled turtle ( Trionyx sinensis) were observed with two affinity histochemical methods, ABC staining and fluorescence bound lectin staining. Concanavalin (ConA), Phytohemagglutinin (PHA), Pokeweed agglutinin (PWM) and Lipopolysaccharide (LPS) binding sites were distributed mainly on the plasma membrane of lymphocytes. ConA, PHA binding sites were distributed in thymus, cordic structure of head kidney and scatterly spleenic tissue of C. idellus, also in thymus and periellipsoidal lymphatic sheaths and marginal region of T. sinensis. PWM, LPS binding sites were scatterly distributed in cordic structure of head kidney of C. idellus, spleenic tissue of C. idellus and T. sinensis, but positive cell groups were not obvious. ConA, PHA and PWM positive percents of lymphocytes in blood smears were repectively 36%~41%, 33%~37%, 38%~43% in C. idellus and 37%~48%, 36%~45%, 44%~53% in T. sinensis, but percents of positive cells were repectively 15%~18%, 14%~17%, 9%~12% in imprints of head kidney of C. idellus.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2001年第3期355-359,T001,共6页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金 (No .3 0 0 70 5 88)
湖北省自然科学基金
中国科学院所长择优基金资助项目&&
关键词
草鱼
中华鳖
淋巴细胞
凝集素结合部位
受体
组织化学
造血器官
Grass carp (Ctenopharyngodon idellus), Chinese soft shelled turtle ( Trionyx sinensis), Lymphocyte, Lectin binding pattern (receptor), Histochemistry