摘要
利用PCR方法分别构建了烟草花叶病毒 (TMV)中一个推测为复制酶的 5 4_kD蛋白基因 (5 4K)缺失N端、C端和仅余基因中部 2 6 1bp的 3个缺失突变体 ,与野生型 5 4K一起克隆入植物中间载体p2 0 8,并通过根癌土壤杆菌(Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化烟草 (NicotianatabacumL .)。用TMV侵染转基因植物的R0 代和R1代 ,结果显示这
Three deletion mutants of tobacco mosaic virus (TMV) 54-kD putative replicase gene (54K) were obtained by PCR, and cloned into plant expression vector p208, then transformed into Nicotiana tabacum L. cv. SR1 by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid-mediated transformation. All the transgenic plants with the N-terminal deletion mutant, the C-terminal deletion mutant and the only 261 nucleotides region from the central part of the 54K ORF showed significant resistance against TMV.
基金
国家"8 6 3"项目!(10 3_13_0 2_0 5 )&&
