摘要
利用微卫星 (SSR)分子标记对福建省内 4个栲树 (CastanopsisfargesiiFranch .)群体遗传结构进行了研究。SSR标记揭示了栲树群体丰富的遗传变异 :平均等位基因数A =9.0 ,平均有效等位基因数Ne=4.8,平均期望杂合度He=0 .6 5 ,而群体具有较低的Fst值 (Fst=0 .0 31)。SSR每个位点的等位基因频率分布在栲树群体间都存在显著或极显著差异 ,表明根据SSR等位基因频率分布亦能了解群体的分化。SSR标记使栲树群体中一些稀有等位基因得以表现 ,5 4个SSR等位基因中有 15个等位基因仅出现在 1个或 2个群体中 ,且频率较低 。
Genetic structure of four populations in Castanopsis fargesii Franch. in Fujian Province was studied with microsatellite (SSR) markers. A high level of genetic variation was detected in the populations of C. fargesii by using SSR with A = 9.0, N-e = 4.8, H-e = 0.65 and the population differentiation coefficient ( F-st) was only 0.031. The distributions of alleles of all loci were significantly different among the populations of C. fargesii, and the population differentiation could be found according to the distributions of SSR alleles. Some rare alleles in the populations of C. fargesii were revealed by SSR: Fifteen of 54 alleles appeared in one or two populations with lower frequencies; conservation of these rare alleles is of great importance.
基金
国家自然科学基金农业倾斜项目!(39770 6 2 8)&&
关键词
微卫星
栲树
群体遗传结构
等位基因频率
SSR
microsatellite
Castanopsis fargesii
population genetic structure
allele frequency
