二氯甲烷降解菌的紫外线诱变及降解工艺条件的研究

Ultraviolet Mutation Breeding of Dichloromethane Degradative Bacteria and Conditions of Degradation

无碳查氏平板上稀释涂布降解菌 ,平板倒置 ,皿盖中央放一块无菌棉花吸附二氯甲烷 ,利用二氯甲烷在培养温度下挥发的特性 ,提供气态碳源供菌生长 ,可获得非水溶性有机废气降解菌的单菌落。二氯甲烷降解菌经鉴定为假单胞菌、盐杆菌和放线菌分枝杆菌。经紫外线诱变处理 ,混合突变株摇瓶降解工艺条件 :2 #培养基、pH7.0、温度 2 5～ 2 8℃、摇床转速 1 0 0转 /分。温度高转速快二氯甲烷挥发多 ,菌体能利用的碳源少从而影响的生长和降解。应用本研究工艺 ,控制进气量小于 1 .5mg/L ,在生物膜填料塔中发大试验 ,二氯甲烷的降解率达 85 %。

Add the dilution degradative bacteria on top of a Petri plate already containing Czapek's medium which didn't contain carbon source.Plates were placed in an inverted position.A piece of sterilized cotton is put on the cover to adsorbed dichloromethane.At culture temperature,dichloromethane volatilize,a gaseous carbon which can make microorganism growth,some colonies can be obtained,Dichloromethane degradative bacteria are Pseudomonas,Halobacterium and Mycobacteriaceae. The degradative bacteria mutate by ultraviolet radiation.The conditions of degradation for mixed mutants in shake flasks are pH about 7.0.temperature about 25～28*!℃;the higher temperature the faster dichloromethane volatilize. 2 # medium is better than Czapek's medium.When the inlet gas content was below 1.5*!mg/L the elimination efficiency maintained above 85% in the biotrickling filter.