兔血管对He-Ne激光的反射、透射、吸收系数和散射相函数的研究

Reflection,Transmission,Absorption Coefficients and Scattering Phase Function of Rabbit Arteries and Veins at He-Ne Laser in Vitro

本文运用光生物学、基础医学以及物理学的基本理论 ,探讨了兔血管组织的激光传播特性 ,定量测定了兔血管组织的激光传输参量。采用标准积分球测量了兔血管组织样品在 632 .8nmHe Ne激光照射下的透射率 (T)和反射率 (R) ,推算吸收率 (A)。并把分光仪改装为光散射测量装置 ,测量了 632 .8nmHe Ne激光照射下 ,- 90°～ 90°散射角范围内的散射相函数S(θ) ,计算平均散射余弦 μ。结果表明 :兔动脉对He Ne激光的漫反射率比静脉的大 (P <0 .0 1 ) ,而透射率却明显比静脉小 (P <0 .0 1 ) ,而动脉的吸收系数明显地比静脉要小 ,He Ne激光对静脉的穿透深度明显比动脉的要大。兔动脉和静脉对He Ne激光有较强的反向散射 (μa<0 .5 ,μν<0 .5) ,且静脉的反向散射明显较动脉的要强 ,其相应的散射相函数S(θ)有着明显的差别。兔动脉和静脉对He Ne激光的反射、透射、吸收系数及散射相函数等光学性质的明显不同提示在应用He Ne激光进行血管疾病的治疗时应加以考虑。

In the present paper,the characteristic of laser light transporting in rabbit arteries and veins with the aid of the primary theories of photobiology,elementary medical science and physics were studied.Diffuse reflectance(R) and transmittance(T) of rabbit arteries and veins samples were measured by a standard integrating spheres system,using He-Ne laser in 632.8*!nm wavelength and the absorbance(A) was deduced.The scattering phase function S(θ) were obtained by the experimental arrangement repacked from a spectroscope at angles from -90° to 90°for rabbit arteries and veins samples in 632.8*!nm wavelength and the mean cosines of scatter(μ) was calculated.The results showed that the diffuse reflectance of rabbit arteries was bigger than that of veins at He-Ne laser(P<0.01).The transmittance of rabbit arteries was obviously lesser than that of rabbit veins(P<0.01).The absorption coefficient of rabbit arteries was obviously lesser than that of veins.The optical penetration depth of rabbit veins was obviously lsser than that of rabbit arteries at He-Ne laser.Rabbit arteries and veins had stronger backward scattering(μ α<0.5,μ ν0.5),and that backward scattering of rabbit veins was obviously stronger than that of rabbit arteries at He-Ne laser.There are obvious difference of the scattering phase functions between rabbit arteries and veins at He-Ne laser.There are obvious difference of the optical properties between rabbit arteries and veins to the reflection,transmission,absoprtion coefficients and scattering phase functions of He-Ne laser.It is prompted that should be considered when He-Ne laser was applied to treat illness of human artery and vein.