摘要
用根癌农杆菌 (Agrobacteriumtumefaciens)菌株C58感染栝楼 (TrichosantheskirilowiiMaxim .)无菌苗诱导冠瘿瘤 ,获得其冠瘿组织 ;栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长 ,纸电泳检测结果表明其合成了冠瘿碱 ,表明Ti质粒转化成功。栝楼冠瘿组织在MS培养基上形成完整植株 ,移栽后良好生长。
Crown galls were induced by direct infection of sterilized seedlings of Trichosanthes kirilowii Maxim. with Agrobacterium tumefaciens C58. Transformation of Ti in C58 was inspected by high_pressure_paper electrophoresis; the detection of nopaline in the crown galls showed that T_DNA of Ti plasmid had been transformed successfully. The crown tissues produced plantlets on MS medium and the plantlets were successfully transplanted and grew well.
出处
《植物学通报》
CSCD
北大核心
2001年第3期363-366,共4页
Chinese Bulletin of Botany
