基因型和激素浓度对大豆植株再生的影响

The Effects of Genotype and Phytohormone Concentration on Soybean Plant Regeneration

本文采用 8个大豆基因型 ,比较了NAA激素浓度对大豆下胚轴植株再生的影响。结果表明 :所有的基因型在合适的培养条件下 ,均能形成愈伤组织 ,但不同基因型由愈伤组织诱导植株再生频率有很大差异。供试的 8个品种中 ,在NAA浓度为 0 .1～ 0 .5mg/L的培养基上 ,汾豆 33号表现出较高的再生率 ,达 50 % ;晋豆 1 9号次之 ,为 42 % ;而晋旱 1 2 5则较低 ,仅 1 9%。较合适的培养条件是 :下胚轴在B5+NAA 0 .3mg/L +KT 1mg/L + 3%蔗糖 ,可高频率地诱导出愈伤组织并直接分化成苗。

Eight soybean genotypes were used to study the effects of genotype and NAA concentration on plant regeneration from hypocotyl explant. All the genotypes used in the test produced calli, but regeneration rate varied greatly between the genotypes. In the 8 tested genotypes, Fen 33 produced the highest regeneration rate, up to 50%; Jin 19 had 42% regeneration; Jinhan 125 was the lowest, with 19% regeneration. The most desirable medium in the test was B 5+ NAA 0.3 mg/L +KT 1 mg/L+3% sucrose.