苯并(a)芘对大弹涂鱼肝脏抗氧化酶活性影响的初步研究

Effect of benzo(a)pyrene on antioxidant enzyme activities in Boleophthalmus pectinirostris liver.

在实验条件下 ,研究了不同浓度苯并 (a)芘 (BaP)暴露对大弹涂鱼肝脏内抗氧化酶 超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶 (GPx)和过氧化氢酶 (CAT)活性的影响 .结果表明 ,不同浓度的BaP暴露对抗氧化酶活性产生不同程度的影响 .低浓度组 (3μg·L-1)BaP暴露 ,抗氧化酶活性未发生显著变化 .高浓度组 (30 μg·L-1)BaP暴露 ,随着污染时间的延长 ,SOD活性在第 3天显著升高 (α≤ 0 .0 5 ) ,随后逐渐降至对照组水平 ;GPx活性则在第 7天显著升高 (α≤ 0 .0 5 ) .在整个实验周期中 ,BaP暴露浓度的变化以及暴露时间的延长对CAT活性无显著影响 .

The effect of different BaP concentrations on the activities of superoxide dismutase(SOD),glutathione peroxidase(GPx) and catalase(CAT) in Boleophthalmus pectinirostris liver was studied under experimental condition.The results showed that the activities of antioxidant enzymes did not change too much with BaP exposure in lower concentration,whereas the SOD and GPx activities were increased significantly with BaP exposure in higher concentration.No statistical changes were observed in the effect of BaP exposure in different concentrations on CAT activities throughout the experiment.