胃癌及癌前病变组织中转化生长因子βⅠ型受体的表达与细胞凋亡

The expression of TGF β receptor Ⅰ and apoptosis in gastric carcinoma and precancerous lesions.

目的 探讨胃癌及癌前病变中转化生长因子 βⅠ型受体 (TGF βRⅠ )的表达与细胞凋亡的关系及它们在胃癌发病机制中的作用。方法 免疫组化SP法检测慢性浅表性胃炎 (30例 )、肠化生 (30例 )、不典型增生 (18例 )及胃癌 (2 5例 )中TGF βRⅠ的表达 ,TUNEL法检测其中凋亡细胞的情况。 结果 从慢性浅表性胃炎到肠化生、不典型增生及胃癌 ,TGF βRⅠ表达及凋亡指数 (AI)分级均与胃粘膜病变程度显著负相关 (分别为 r =-0 72 72 ,P <0 0 1;r =- 0 8347,P <0 0 1) ,19例胃癌 (76 % )TGF βRⅠ表达缺失 ;不同组织学类型中AI的分布和TGF βRⅠ表达的分布差异无显著性 (P =0 0 779)。结论 TGF βRⅠ表达缺失可能影响细胞凋亡 ,参与胃癌的发生。

Objective To investigate the relationship between the expression of TGF β receptor Ⅰ(RⅠ)and apoptosis in gastric carcinoma and precancerous lesions,and their effects in the development of gastric carcinoma.Methods The expressions of TGF βRⅠ in 103 cases,including CSG(30 cases),IM(30 cases),Dys(18 cases)and GAC(25 cases)were detected by immunohistochemical techniques(SP),apoptosis cells were examined by terminal deoxynucleotidyl transferase(TdT) mediated dUTP nick end labelling(TUNEL).Results Both the expressions of TGF βRⅠ and apoptosis indexes(AI:percentage of TUNEL positive cells)showed negative correlation with the degree of gastric mucosa lesions from CSG,IM,Dys to GAC(r=-0\^7272,P<0\^01;r=-0\^8347,P<0\^01,respectively).19 cases of GAC(76%)did not express TGF βRⅠ.There was no significant difference between the distribution of AI and the expression of TGF βRⅠ in different histological types(P=0\^0779).Conclusion The loss of TGF βRⅠ expression may play an important role in the inhibition of TGF βRⅠ induced apoptosis and be involved in the development of gastric carcinoma.