摘要
目的 :研究正常乳腺上皮细胞与乳腺癌细胞之间以及不同恶性程度的乳腺癌细胞之间的细胞周期相关因子的表达异同。方法 :采用Western印迹法检测正常乳腺细胞株AG11132A、ER阳性的乳腺癌细胞株MCF 7及ER阴性的乳腺癌细胞株MDA MB 2 31之间细胞周期蛋白D1、E及P2 1蛋白表达的异同及与其生物学特性的关系。结果 :(1)正常乳腺上皮细胞株高表达P2 1蛋白 ,低表达周期蛋白E。与正常细胞相比 ,乳腺癌细胞株MCF 7、MDA MB 2 31细胞高表达周期蛋白E ,其中高表达的周期蛋白E中存在异常的低分子量周期蛋白E成分 ,而正常乳腺上皮细胞不表达这种异常的周期蛋白E。 (2 )在乳腺癌细胞株之间 ,相对ER阳性的MCF 7细胞 ,ER阴性的MDA MB 2 31细胞则高表达周期蛋白E ,基本无表达P2 1蛋白。结论 :(1)正常细胞与癌细胞之间、相对低恶性程度的癌细胞与相对高恶性程度的癌细胞之间的差别是多环节的、质和量的异常导致的结果 ;(2 )周期蛋白E及P2 1均可反映乳腺癌细胞的增殖活性和恶性程度 。
Purpose:To clarify the different expression patterns of the cyclins and P21 between the normal breast epithelium cells and breast cancer cells of different degrees of malignancy.Methods:Using Western Blot, we detected the protein expression of cyclin D1, E, and p21 in the normal breast epithelium cells AG11132A, ER positive MCF 7 breast cancer cells and ER negative MDA MB 231 breast cancer cells.Results:(1) P21 protein was highly expressed and cyclin E lowly expressed in the normal breast epithelium cells. Compared with the normal breast epithelium cells, Cyclin E were highly expressed in breast cancer cells of MCF 7 and MDA MB 231 cells. Abnormal low molecular weight cyclin E was co expressed with normal cyclin E in both MCF 7 and MDA MB 231 breast cancer cells, but not in normal breast epithelium cells AG11132A. (2) In breast cancer cells, compared with the ER positive MCF 7 cells the ER negative MDA MB 231 cells expressed a high level of cyclin E protein but low p21.Conclusions:(1) There are many forms of differences both quantitive and qualitative between normal cells and cancer cells, as well as between relatively low degree and high degree invasive malignant cancer cells. (2) Cyclin E and P21 may be potential indicators that reflect the malignancy of breast cancer cells, but they should be analyzed as a whole in order to get more accurate prognostic information. [
出处
《中国癌症杂志》
CAS
CSCD
2001年第3期193-195,共3页
China Oncology
基金
国家自然科学基金 (编号 :3 9670 80 3 )
