幽门螺杆菌感染与长乐高发区胃癌发生的关系

The relationship between the infection of Helicobacter pylori in gastric mucosa and the gastrocarcinogenesis in Changle city

目的 :探讨胃粘膜幽门螺杆菌 (Helicobacterpylori,Hp)感染对细胞凋亡及EGFR、VEGF表达的影响 ,从而初步探讨Hp感染与长乐市高发区胃癌发生的关系。方法 :采用碱性品红染色法和血Hp抗体检测法检测Hp感染状况 ;原位末端标记法 (TUNEL法 )检测胃粘膜细胞凋亡指数 ;免疫组化S P法检测胃粘膜EGFR及VEGF表达情况。结果 :长乐市胃癌演化系列Hp现症感染率 (Hp +率 )为 74.4% ,以慢性萎缩性胃炎伴或不伴肠化 (CAG IM)组最高 ,为 92 .5 % ;细胞凋亡指数在CAG IM组最高 ,为 0 .35 7,而胃癌组最低 ,为 0 .179;EGFR表达阳性率为 5 1.35 % ,其中CAG IM组阳性率最高 ,达 75 .0 % ,慢性浅表性胃炎 (CSG)组最低 ,为 2 5 .0 % ,胃癌组为 46 .7% ;VEGF在不典型增生 (AH)组最低 ,为 35 .0 % ,在胃癌组最高 ,为 6 5 .0 %。 (P <0 .0 5 )。VEGF表达与凋亡指数呈负相关 (P <0 .0 5 )。Hp感染使胃粘膜凋亡指数增高 ,EGFR表达增加 ,VEGF表达下降 (P <0 .0 5 )。结论 :Hp感染与长乐市高发区胃癌发生相关 ,Hp感染影响了胃粘膜细胞EGFR、VEGF的表达及胃粘膜细胞的增生和凋亡 ,从而可能影响了胃癌演化系列发生。

Purpose:To explore the relationship between the infection of Helicobacter pylori (Hp), cell apoptosis and the expression of EGFR,VEGF in gastric mucosa and the gastrocarcinogenesis in Changle city, where has a high incidence of gastric carcinomas. Methods:The status of Hp infection was detected by the serum Hp antibody detection and the Hp special staining. The in situ TUNEL method was used in the apoptosis index (AI) detection. The immunohistochemical method (with S P kit) was used in the detection of the EGFR, VEGF expression. Results:The present infection rate of Hp in the series of the gastrocarcinogenesis was 74.4%, and was highest in the objects with chronic atrophic gastritis and/or intestinal metaplasia (CAG IM, 92.5%) . The AI was highest in the subjects with CAG IM, and lowest in the subjects with chronic superficial gastritis (CSG), the data were 0.357 and 0.179 respectively. The expression rate of EGFR was highest in the subjects with CAG IM, and lowest in the subjects with CSG, the data were 51.35% and 75% respectively. The expression rate of VEGF was highest in the subjects with GC, and lowest in the subjects with AH, the data were 65% and 35% respectively ( P <0.05). The VEGF expression was negatively related with the AI ( P <0.05). The infection rate of Hp was related with the higher AI, the higher EGFR expression and lower VEGF expression. ( P <0.05). Conclusions:The Hp infection is associated with the gastrocarcinogenesis in Changle city. The serial change of the AI and the EGFR, VEGF expression in the series of gastrocarcinogenesis, which are related with Hp infection, influences the process of the occurrence and development of gastric carcinoma, and may possibly promote gastrocarcinogenesis. [