大肠癌中PCNA、p53和C-erbB-2的表达及与机体免疫反应的关系

Expression of PCNA、p53、C-erbB-2 in colorectal carcinoma and its relationship with host immune reactions

目的 :观察大肠癌和癌旁粘膜中PCNA、p5 3和C erbB 2的表达及与机体免疫反应的关系。方法 :用免疫组化Envision法检测 42例大肠癌组织、35例癌旁及 10例正常粘膜中PCNA、p5 3和C erbB 2的表达 ,观察切片中纤维增生、单个核细胞浸润、淋巴结反应情况 ,以反映机体的免疫反应 ,并分析与PCNA、p5 3和C erbB 2表达的关系。结果 :(1)大肠癌中PCNA、p5 3和C erbB 2的表达显著高于正常大肠粘膜 ,且癌旁组织PCNA的强阳性率高于p5 3和C erbB 2 (P <0 .0 5 )。 (2 )p5 3强阳性者淋巴结生发中心 (GH)增生明显减少 (P <0 .0 5 ) ,淋巴结转移增加。而纤维增生和单个核细胞浸润无明显变化 (P >0 .0 5 )。PCNA和C erbB 2阳性强度在机体免疫反应指标中无明显变化 (P >0 .0 5 )。 (3)淋巴结生发中心 (GH)增生比副皮质区 (PH)增生明显 ,且GH增生者淋巴结转移明显减少 (P <0 .0 5 )。结论 :(1)PCNA、p5 3和C erbB 2联检可作为筛选大肠癌的参考指标。 (2 )机体免疫反应指标 (尤其是GH增生 )和p5 3的检测可作为判断大肠癌转移和预后的参考指标。

Purpose:To investigate the expression of PCNA、p53、C erbB 2 in colorectal carcinoma and its relationship with host immune reactions. Methods:The expression of PCNA、p53、C erbB 2 was detected by Envision immunohistochemical method in 42 colorectal carcinoma, 35 pericarcinomatous and 10 normal coloerctal mucosa respectively. Host immune reactions (pericarcinomatous fibrosis, mononuclear infiltration and lymph nodes reaction) were also observed. Results:(1) The positive expression of PCNA、p53、C erbB 2 in colorectal carcinoma was significantly higher than in corresponding pericarcinomatous mucosa and normal colorectal mucosa respectively( P <0.05). (2) The positive expression of p53 was related to the high rate of lymph node metastasis and had reverse relationship with host immune reaction such as GH( P <0.05), but was not related to fibrosis and mononuclear infiltration ( P >0.05). (3) GH was more evident than PH in colorectal carcinoma and GH had reverse relationship with lymph node metastasis( P <0.05). Conclusions:(1) Serial tests of PCNA、p53、C erbB 2 could be used as the markers to screen colon carcinoma. (2) The detection of host immune reaction (particularly GH) and p53 protein expression may be valuable in evaluation lymph node metastasis and prognosis of colon carcinoma.[