不同剂量三氧化二砷对食管癌细胞的作用

The Effects of Arsenite Trioxide in Various Concentrations on the Esophageal Carcinoma Cell Line

目的 :研究不同浓度As2 O3对食管癌细胞系的作用。方法 :食管恶性转化上皮细胞 (SHEEC1)是我室用人乳头状瘤病毒 (HPV) 18和TPA诱发的细胞系 ,培养在培养瓶和 2 4孔板 ,用浓度 1,3和 5 μmol/L的As2 O3处理 2 ,4 ,8,16,2 4h ,电镜检查细胞形态 ,光镜检查核分裂指数 (MI) ;用放射自显影检查 [3H] TdR掺入细胞 ;流式细胞仪检查增殖指数 (PI)和凋亡指数 (AI)。结果 :低剂量As2 O3( 1.0 μmol/L)组 [3H] TdR掺入细胞 ,MI,PI皆增加 ,提示SHEEC1增殖率提高 ;高剂量组 ( 3和 5 μmol/L)高AI和低PI,给药 2 4h后 ,电镜可见细胞凋亡和坏死。结论 :不同剂量氧化砷的作用不同 ,低剂量可引起食管癌细胞DNA合成增加和促进细胞增殖 ,高剂量则引起细胞凋亡和坏死。

Objective: To further study the effects of As 2 O 3 in various concentrations on the esophageal carcinoma cell line. Methods: The esophageal malignant transformed epithelial cells (SHEEC1) were induced by HPV18 in synergy with TPA in our laboratory. The cells cultured in flask and 24 wells plate were treated by As 2 O 3 with concentrations of 1, 3 and 5 μmol/L for 2, 4, 8, 16, 24 h respectively. The morphologic changes of cells were observed under election microscopy. The mitotic index (MI) of living cells was calculated by phase contrast microscopy and the cells with TdR uptake were examined by autoradiography. The proliferative index (PI) and apoptotic index (AI) were assayed by flow cytometry. Results: A low dosage of As 2 O 3 (1.0 μmol/L) enhancing the protiferative rate of SHEEC1 was demonstrated with TdR uptake, MI and PI increased. The high AI and low PI were found in the high concentrations (3 and 5 μmol/L)of As 2 O 3 . The morphological changes of apoptosis and necrosis were found in 24 h after As 2 O 3 in high concentrations (3 and 5 μmol/L) administrated. Conclusion: The effects of As 2 O 3 in various concentrations are different. Low concentration of As 2 O 3 promotes the proliferation of the esophageal carcinoma cells by increment of DNA synthesis. In high concentration of As 2 O 3 apoptosis and necrosis are induced.