接种9724肝癌细胞后不同免疫力小鼠体内T细胞的作用

The Effects of T Lgmphocytes in Various Strains of Mice in vivo after Inoculation with Hepatocarcinoma Cell Line 9724

目的 :探讨人类肝癌细胞系 972 4在不同免疫力小鼠中的生长特点及T细胞对癌细胞的排斥特点。方法 :体外培养 972 4 ,接种到不同免疫力小鼠 (正常BALB/c ,B细胞缺陷的CBA/N ,T细胞缺陷的BALB/c nu ,T细胞、B细胞缺陷的SICD小鼠及免疫重建的SCID小鼠 )中 ,观察其生长特性 ;测定小鼠脾细胞杀伤力流式细胞仪测定外周血CD4 +,CD8+的百分率。结果 :BALB/c小鼠和免疫重建的BALB/c PBL SCID不成瘤 ;CBA/N随接种数量和途径不同而有不同的表现 ;BALB/c nu ,SCID小鼠和免疫重建的CBA/N PBL SCID10 0 %成瘤。接种过癌细胞的BALB/c和CBA/N小鼠脾细胞对癌细胞杀伤力较强 ,免疫重建的SCID杀伤力较小。不同组别CD4 +百分率都下降 ,CD8+变化不大 ,CD4 +/CD8+比值下降。结论 :小鼠成癌率与T细胞的作用最为密切 ;

Objective: To explore the charateristics of human hepatocarcinoma cells growing in various strains of mice with different immunity and T lymphocyte rejection to the hepatocarcinoma cells. Methods: The human hepatocarcinoma cells were inoculated into various strains of mice with different immunity (including BALB/c, CBA/N, BALB/c nu, SCID and BALB/c PBL SCID,CBA/N PBL SCID) and their growths were surveyed. Then the spleen cells were harvested to examine their direct cytotoxicity to the hepatocarcinoma cells.The percentage of CD4 + and CD8 + cell of peripheral blood from mice was assyed by FCM. Results: There was no carcinoma growth in BALB/c and BALB/c PBL SCID,but was 100% carcinoma growth in SICD, nude and CBA/N PBL SCID. The spleen cells in experimental groups from BALB/c and CBA/N mice displayed strong cytotoxicity to target cells and those of immunoreconstituted SCID generated a little lysis to target cells, but those of control group and nude, SCID mice generated no lysis to target cells.The percentage of CD4 + from BALB/c and CBA/N mice in experimental groups decreased,but the percentage of CD8 + changed not much while CD4 + /CD8 + ratio decreased.Couclusion: Whether the hepatocarcinoma cells grew in mice correlated strongly with T lymphocytes which were xenospecific cytotoxicity and played a main role of immune killing in rejection to xenografted tumor.