整合素α_4β_1及其配体与大鼠肝肿瘤周边肥大细胞募集的关系

Correlation of integrinα_4β_1 and its two ligands with mast cell recruitment in the surroundings of rat liver tumor

目的 :研究整合素α4 β1(VLA 4)及其配体VCAM 1(vascularcelladhesionmolecular 1)和FN(fibronectin)与肝肿瘤周边肥大细胞 (mastcell,MC)募集的关系。方法 :根据肝肿瘤周边肥大细胞数量 ,将 18只雄性Wistar大鼠移植肝肿瘤模型进行分组 ,8只正常雄性Wistar大鼠作对照。用间接免疫荧光和流式细胞术检测各组大鼠腹腔肥大细胞整合素VLA 4分子的表达水平 ,同时用免疫组化研究肿瘤周边肝组织血管内皮细胞和肝窦内皮细胞表面VCAM 1和肿瘤周边FN的表达。结果 :不同肝肿瘤大鼠肿瘤周边浸润肥大细胞数量有明显差异。各组大鼠腹腔MC表达整合素VLA 4分子均呈阳性 ,肿瘤周边肥大细胞浸润较多组 ,其整合素α4 β1表达水平也较高。肿瘤周边血管内皮和窦内皮细胞表达VCAM 1阳性。肿瘤周边沉积大量呈阳性表达的FN与肥大细胞紧密相联。结论 :整合素α4 β1及其配体VCAM 1和FN在肝肿瘤周边肥大细胞募集中起重要作用 ;整合素α4

Purpose To study the correlation of integrinα 4β 1 and its two ligands (vascular cell adhesion molecule 1 and fibronectin)with mast cell(MC)recruitment around the rat hepatocarcinoma. Methods Eighteen male Wistar rats with liver tumor were divided into three different groups in terms of mast cell numbers in the surroundings of liver tumor. Eight normal Wistar rats were used as control. Integrin VLA 4 expression in rat peritoneal mast cells was analyzed by indirect immunofluorescence and flow cytometry. Immunohistochemistry was also used to investigate whether endothelial cell VCAM 1 and fibronection were expressed positively. Results There were markedly different in mast cell numbers around rat liver tumor. Mast cells express high levels of integrin α 4β 1 on their surfaces. And the more mast cells around liver tumor, the higher levels of integrin VLA 4. We also found that endothelial cells expressed VCAM 1 and there are a number of fibronectin deposition around rat hepatocarcinoma. Conclusions Integrin α 4β 1/VCAM 1 and fibronectin play an important role in mast cell recruitment mechanism around liver tumor. The expression levels of integrin α 4β 1 are paralleled by mast cell accumulation in the surroundings of hepotocarcinoma.