肝素对鼠肺成纤维细胞胶原mRNA表达的影响

Effects of heparin on expression of collagen mRNA in rat lung fibroblast

目的 :探讨不同剂量的肝素对胎鼠肺成纤维细胞 (LFb)Ⅰ、Ⅲ型胶原mRNA表达的影响。方法 :采用Northern印迹杂交方法检测Ⅰ、Ⅲ型胶原mRNA的表达。结果 :浓度为 0 1mg/L、1mg/L、10mg/L的肝素对Ⅰ、Ⅲ型胶原mRNA的表达的影响不明显 ;10 0mg/L的肝素则抑制Ⅰ、Ⅲ型胶原mRNA的表达。结论 :Ⅰ。

Purpose To study the effect of heparin on expression of type collagen Ⅰ,ⅢmRNA in cultured fetal rat lung fibroblast (LFb). Methods By using Northern blot method and immage analysis,the expression of collagenⅠ, Ⅲ mRNA were quantitatively analysed in the fibroblasts treated with different concentration heparin (0 1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L)separately. Results Heparin treatment at 0 1 mg/L,1 mg/L, 10 mg/L and did not influence the expression of collagen Ⅰ,Ⅲ mRNA,but the expression of collagen Ⅰ,Ⅲ mRNA were inhibited at 100 mg/L. Conclusion Expression of collagen Ⅰ, ⅢmRNA is related to the concentration of heparin.