左旋咪唑对实验性过敏性脑脊髓炎大鼠脊髓IL-1、IL-2及IL-6 mRNA表达的影响

The Effects of Levamisole on the Expressions of IL-1,IL-2 and IL-6 mRNA in the Spinal Cord of Rats with Experimental Allergic Encephalomyelitis

目的 了解左旋咪唑 (LMS)对实验性过敏性脑脊髓炎 (EAE)大鼠脊髓内白细胞介素 (IL) 1、IL 2及IL 6mRNA表达的影响 ,为探讨LMS性白质脑病的发病机制提供实验依据。方法 用豚鼠全脊髓匀浆免疫Wistar大鼠建立EAE模型 ,EAE +LMS组大鼠分别于免疫后 0、2 4、48hipLMS 10mg/kg ;大鼠免疫后第 16天处死 ,采用RT PCR的方法检测大鼠脊髓内IL 1α、IL 1β、IL 2和IL 6mRNA表达的相对量。 结果 和正常对照组相比 ,EAE组大鼠脊髓内IL 1α(P <0 .0 5 )、IL 1β(P <0 .0 5 )、IL 2 (P <0 .0 1)和IL 6 (P <0 .0 5 )mRNA的表达量均明显升高。EAE +LMS组大鼠脊髓内IL 1α、IL 1β、IL 2和IL 6mRNA的表达量和EAE组相比则分别升高 131% (P <0 .0 5 )、12 1% (P <0 .0 5 )、95 % (P <0 .0 5 )和 2 8% (P >0 .0 5 )。结论 LMS能明显促进EAE大鼠脊髓内IL 1α、IL 1β、IL 2mRNA的表达 。

Purpose: To study the effects of levamisole(LMS) on the expressions of interleukin(IL)-1α, IL-1β, IL-2 and IL-6 mRNA in the spinal cord of rats with experimental allergic encephalomyelitis(EAE) in order to investigate the mechanism of leukoencephalopathy associated with LMS. Methods: The EAE induced by the homogenate of spinal cords from guinea pigs with complete Freund's adjuvant(CFA). The intraperitoneal injection of LMS with a dose of 10 mg/kg was given to the rat of EAE + LMS group at 0.24, 48 h respectively after immunization. All rats were killed 16 days post immunization. The expressions of IL-1α, IL-1β, IL-2, and IL-6 mRNA were assayed by semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR). Results: Compared with control group, IL-1αa(P 0.05) compared with levels in rats of EAE group. Conclusions: LMS can remarkably increasa the expressions of IL-1α, IL-1β and IL-2 mRNA in spinal cord of rats with EAE. The results suggest that excess IL-1 and IL-2 play a pathogenic role in the exacerbating effects of LMS on the EAE.