金属离子对蚯蚓CAT、GSH-Px及SOD酶活性的影响

Effect of Metal Ions on Activities of CAT, GSH-Px and SOD from Earthworms, Eisenia Foelide

本文研究了几种金属离子在体内、外对蚯蚓 CAT、GSH- Px及 SOD酶活性的影响。实验结果表明 ,Zn2 + 、Fe3+ 、Mn2 + 在体外对蚯蚓 CAT有抑制作用、Mg2 +、Hg2 +、Cd2 +则对其有激活作用 ,但在体内 Zn2 +、Fe3+则转变呈激活作用 ;Zn2 +、Fe3+、Mn2 +等在体外对蚯蚓 GSH- Px呈抑制作用 ,在体内2 4 h对其也呈抑制作用 ,但 48h却表现为激活作用 ;Zn2 +、Fe3+、Mn2 +、Hg2 +等在体外对蚯蚓 SOD有强烈的抑制作用 ,抑制率达 0 %～ 30 % ,但在体内却表现为不同程度的激活作用。

This paper reported the effects of metal ions on activities of catalase(CAT),glutathione peroxidase (GSH Px)and superoxide dismutase (SOD)from earthworms. The experiment results indicated that catalytic activity is inhibited by Zn 2+ 、Fe 3+ and Mn 2+ and is activated by Mg 2+ 、Hg 2+ 、Cd 2+ in vitro, but the catalytic activity is activated by Zn 2+ 、Fe 3+ in vivo. The glutathione peroxidase activity is inhibited by Zn 2+ 、Fe 3+ et al in vitro and is also inhibited by Zn 2+ 、Fe 3+ 、Mn 2+ in vivo for 24 hour, but change to activation in vivo for 48 hour. The superoxide dismutase activity is strongly inhibited by Zn 2+ 、Fe 3+ 、Mn 2+ and Hg 2+ in vitro and the inhibition ratio is about 30%～0%,but show activation in vivo in varying degress.